Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer

Vet Microbiol. 2014 Jun 25;171(1-2):66-73. doi: 10.1016/j.vetmic.2014.03.009. Epub 2014 Mar 18.


There are few reports describing diarrhea of adult cattle caused by group A rotaviruses. Here, we report the identification of a novel bovine group A rotavirus from diarrhea of adult cows. A group A rotavirus was detected from an epizootic outbreak of diarrhea in adult cows with a decrease in milk production in Japan in 2013. The comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that it had an unreported genotype combination G15P[14]. The genome constellation of this strain, namely, RVA/Cow-wt/JPN/Tottori-SG/2013/G15P[14] was G15-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3 representing VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5, respectively. Each gene segment of Tottori-SG was most closely related to Japanese bovine group A rotaviruses suggesting that Tottori-SG might have derived from multiple reassortment events from group A rotavirus strains circulating among Japanese cattle. No other diarrhea pathogen of adult cattle was detected by routine diagnosis and metagenomics. Viral metagenomics, using a next-generation sequencer, is useful to characterize group A rotaviruses from fecal samples and offers unbiased comprehensive investigations of pathogen.

Keywords: Adult cow diarrhea; Japan; Novel genotype; Rotavirus; Viral metagenomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cattle Diseases / virology*
  • Diarrhea / veterinary*
  • Diarrhea / virology
  • Feces / virology*
  • Female
  • Genome, Viral / genetics
  • Genotype
  • Japan
  • Molecular Sequence Data
  • Phylogeny*
  • Rotavirus / classification*
  • Rotavirus / genetics*
  • Rotavirus Infections / veterinary*
  • Rotavirus Infections / virology
  • Sequence Analysis, DNA / instrumentation
  • Sequence Homology

Associated data

  • GENBANK/AB853890
  • GENBANK/AB853891
  • GENBANK/AB853892
  • GENBANK/AB853893
  • GENBANK/AB853894
  • GENBANK/AB853895
  • GENBANK/AB853896
  • GENBANK/AB853897
  • GENBANK/AB853898
  • GENBANK/AB853899
  • GENBANK/AB853900