Functional consequences of splicing of the antisense transcript COOLAIR on FLC transcription

Mol Cell. 2014 Apr 10;54(1):156-165. doi: 10.1016/j.molcel.2014.03.026.


Antisense transcription is widespread in many genomes; however, how much is functional is hotly debated. We are investigating functionality of a set of long noncoding antisense transcripts, collectively called COOLAIR, produced at Arabidopsis FLOWERING LOCUS C (FLC). COOLAIR initiates just downstream of the major sense transcript poly(A) site and terminates either early or extends into the FLC promoter region. We now show that splicing of COOLAIR is functionally important. This was revealed through analysis of a hypomorphic mutation in the core spliceosome component PRP8. The prp8 mutation perturbs a cotranscriptional feedback mechanism linking COOLAIR processing to FLC gene body histone demethylation and reduced FLC transcription. The importance of COOLAIR splicing in this repression mechanism was confirmed by disrupting COOLAIR production and mutating the COOLAIR proximal splice acceptor site. Our findings suggest that altered splicing of a long noncoding transcript can quantitatively modulate gene expression through cotranscriptional coupling mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Dealkylation
  • Feedback, Physiological
  • Flowers / genetics
  • Flowers / metabolism
  • Gene Expression Regulation, Plant*
  • Histones / metabolism
  • MADS Domain Proteins / genetics
  • MADS Domain Proteins / metabolism*
  • Mutation
  • RNA Splicing*
  • RNA, Antisense / metabolism*
  • RNA, Long Noncoding / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Seedlings / genetics
  • Seedlings / metabolism
  • Time Factors
  • Transcription, Genetic*


  • Arabidopsis Proteins
  • FLF protein, Arabidopsis
  • Histones
  • MADS Domain Proteins
  • RNA, Antisense
  • RNA, Long Noncoding
  • RNA-Binding Proteins