The V domain of dog PVRL4 (nectin-4) mediates canine distemper virus entry and virus cell-to-cell spread

Virology. 2014 Apr;454-455:109-17. doi: 10.1016/j.virol.2014.02.014. Epub 2014 Feb 28.

Abstract

The entry of canine distemper virus (CDV) is a multistep process that involves the attachment of CDV hemagglutinin (H) to its cellular receptor, followed by fusion between virus and cell membranes. Our laboratory recently identified PVRL4 (nectin-4) to be the epithelial receptor for measles and canine distemper viruses. In this study, we demonstrate that the V domain of PVRL4 is critical for CDV entry and virus cell-to-cell spread. Furthermore, four key amino acid residues within the V domain of dog PVRL4 and two within the CDV hemagglutinin were shown to be essential for receptor-mediated virus entry.

Keywords: CDV; Canine distemper virus; Epithelial cell; Hemagglutinin; Nectin-4; PVRL4; Poliovirus receptor-like protein-4; Receptor; Syncytia formation; V domain; Virus entry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion Molecules / metabolism*
  • Distemper Virus, Canine / physiology*
  • Dogs
  • Hemagglutinins, Viral / metabolism
  • Host-Pathogen Interactions*
  • Receptors, Virus / metabolism*
  • Virus Internalization*

Substances

  • Cell Adhesion Molecules
  • Hemagglutinins, Viral
  • Receptors, Virus