Fly-FUCCI: A versatile tool for studying cell proliferation in complex tissues

Cell Rep. 2014 Apr 24;7(2):588-598. doi: 10.1016/j.celrep.2014.03.020. Epub 2014 Apr 13.


One promising approach for in vivo studies of cell proliferation is the FUCCI system (fluorescent ubiquitination-based cell cycle indicator). Here, we report the development of a Drosophila-specific FUCCI system (Fly-FUCCI) that allows one to distinguish G1, S, and G2 phases of interphase. Fly-FUCCI relies on fluorochrome-tagged degrons from the Cyclin B and E2F1 proteins, which are degraded by the ubiquitin E3-ligases APC/C and CRL4(Cdt2), during mitosis or the onset of S phase, respectively. These probes can track cell-cycle patterns in cultured Drosophila cells, eye and wing imaginal discs, salivary glands, the adult midgut, and probably other tissues. To support a broad range of experimental applications, we have generated a toolkit of transgenic Drosophila lines that express the Fly-FUCCI probes under control of the UASt, UASp, QUAS, and ubiquitin promoters. The Fly-FUCCI system should be a valuable tool for visualizing cell-cycle activity during development, tissue homeostasis, and neoplastic growth.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anaphase-Promoting Complex-Cyclosome / genetics
  • Anaphase-Promoting Complex-Cyclosome / metabolism
  • Animals
  • Cell Cycle*
  • Cell Line
  • Cell Proliferation*
  • Cyclin B / genetics
  • Cyclin B / metabolism
  • Drosophila / cytology*
  • Drosophila / genetics
  • Drosophila / metabolism
  • E2F1 Transcription Factor / genetics
  • E2F1 Transcription Factor / metabolism
  • Microscopy, Fluorescence / methods*
  • Organ Specificity
  • Ubiquitination*


  • Cyclin B
  • E2F1 Transcription Factor
  • Anaphase-Promoting Complex-Cyclosome