Assessment of microRNAs in patients with unstable angina pectoris

Eur Heart J. 2014 Aug 14;35(31):2106-14. doi: 10.1093/eurheartj/ehu151. Epub 2014 Apr 11.


Aims: While cardiac troponin measurements have significantly improved the early diagnosis of myocardial infarction, the timely biomarker-based diagnosis of unstable angina pectoris (UAP) remains a major unmet clinical challenge. The aim of this study was to assess levels of circulating microRNAs (miRNAs) as possible novel biomarkers in patients with UAP.

Methods and results: A three-phase approach was conducted, comprising (i) profiling of miRNAs in patients with UAP and controls groups; (ii) replication of significant miRNAs in an independent patient cohort, (iii) validation of a multi-miRNAs panel in a third cohort. Out of 25 miRNAs selected for replication, 8 miRNAs remained significantly associated with UAP. In a validation phase, a miRNA panel including miR-132, miR-150, and miR-186 showed the highest discriminatory power [area under the receiver-operating-characteristic curve (AUC): 0.91; CI: 0.84-0.98].

Conclusion: Using a profiling-replication-validation model, we identified eight miRNAs, which may facilitate the diagnosis of UAP.

Keywords: Acute myocardial infarction; Circulating microRNA; Diagnosis; Myocardial infarction; Unstable angina pectoris.

Publication types

  • Evaluation Study
  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Angina, Unstable / diagnosis*
  • Case-Control Studies
  • Early Diagnosis
  • Female
  • Genetic Markers
  • Genetic Techniques
  • Humans
  • Male
  • MicroRNAs / metabolism*
  • Middle Aged
  • Myocardial Infarction / diagnosis
  • ROC Curve
  • Reproducibility of Results


  • Genetic Markers
  • MicroRNAs