Characterization of the gene for prostate-specific antigen, a human glandular kallikrein

Biochem Biophys Res Commun. 1989 Jun 30;161(3):1151-9. doi: 10.1016/0006-291x(89)91362-4.

Abstract

The gene for the human glandular kallikrein, prostate-specific antigen, has been cloned. The sequence of 7130 nucleotides encompassing the gene and 633 bp of 5' and 639 bp of 3' flanking DNA has been determined. The translation initiation site was slightly heterogeneous, yielding 5' non-translated leader sequences of 41 and 35 bp. The gene is divided into five exons, with introns located at positions identical with those found in other glandular kallikrein genes. The nucleotide sequence is very similar to that of the human kallikrein gene hGK-1, with 76 to 93% of the nucleotides being identical in the exons and 76 to 87% in the introns. The similarity also extends approximately 200 bp into the sequence flanking the 5' end of hGK-1 and several other, both human and rodent, glandular kallikrein genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Neoplasm / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Exons
  • Genes*
  • Humans
  • Introns
  • Kallikreins / genetics*
  • Male
  • Molecular Sequence Data
  • Prostate / immunology*
  • Prostate-Specific Antigen
  • RNA Caps / genetics
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Tissue Kallikreins

Substances

  • Antigens, Neoplasm
  • RNA Caps
  • Kallikreins
  • Tissue Kallikreins
  • Prostate-Specific Antigen

Associated data

  • GENBANK/M27274