RUVBL1 directly binds actin filaments and induces formation of cell protrusions to promote pancreatic cancer cell invasion

Int J Oncol. 2014 Jun;44(6):1945-54. doi: 10.3892/ijo.2014.2380. Epub 2014 Apr 10.


We report a novel function of RUVBL1 molecule in pancreatic cancer cells. Previous reports describe that RUVBL1 belongs to the family of AAA+ ATPases that associate with chromatin-remodelling complexes and have important roles in transcriptional regulation, the DNA damage response, telomerase activity and cellular transformation. We show that knockdown of RUVBL1 inhibited the motility and invasiveness of pancreatic cancer cells. RUVBL1 localized in the cytoplasm bound filamentous actin (F-actin) in cell protrusions, and increased concentration of monomeric globular-actin (G-actin) in cell protrusions of migrating pancreatic cancer cells. Cytoplasmic RUVBL1 functioned in additional formation of actin filaments in cell protrusions. Consequently, cytoplasmic RUVBL1 contributed to the formation of membrane protrusions by promoting peripheral actin polymerization. Our results imply that these RUVBL1-actin interactions could enhance the invasive properties of pancreatic cancer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATPases Associated with Diverse Cellular Activities
  • Actins / metabolism*
  • Carcinoma, Pancreatic Ductal / metabolism
  • Carcinoma, Pancreatic Ductal / pathology*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Movement / physiology
  • Cell Surface Extensions / metabolism*
  • Cell Surface Extensions / pathology
  • Cytoplasm / metabolism
  • DNA Helicases / genetics
  • DNA Helicases / metabolism*
  • Gene Knockdown Techniques
  • Humans
  • Neoplasm Invasiveness / pathology*
  • Pancreatic Neoplasms / metabolism
  • Pancreatic Neoplasms / pathology*
  • Polymerization


  • Actins
  • Carrier Proteins
  • ATPases Associated with Diverse Cellular Activities
  • DNA Helicases
  • RUVBL1 protein, human