hGK-1: a kallikrein gene expressed in human prostate

Clin Exp Pharmacol Physiol. 1989 Apr;16(4):345-51. doi: 10.1111/j.1440-1681.1989.tb01570.x.


1. Humans have three kallikrein genes: hGK-1, hRKALL (kinin-generating) and a gene encoding prostate specific antigen (PSA). 2. Using a gene-specific oligonucleotide, expression of hGK-1 was demonstrated in human prostate, but not in 10 other tissues examined. 3. The similarity in tissue specificity of expression of hGK-1 with that of the PSA gene led to an examination of putative regulatory DNA. Marked identity of greater than 90% was seen (compared with 80% for hRKALL), thus supporting a role for the operation of similar tissue-specific mechanisms for the control of hGK-1 and the PSA gene. 4. Unique structural features were observed in the protein encoded by hGK-1 suggestive of a capacity for interaction with substrate residues larger than those in kininogen. In particular it is predicted that a glycine at 186 would twist the polypeptide backbone in this crucial, highly conserved active site region and so profoundly influence interaction with substrate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / genetics*
  • Blotting, Northern
  • DNA / genetics
  • Gene Expression Regulation*
  • Humans
  • Kallikreins / genetics*
  • Male
  • Prostate / immunology*
  • Prostate-Specific Antigen
  • RNA / genetics


  • Antigens, Neoplasm
  • RNA
  • DNA
  • Kallikreins
  • Prostate-Specific Antigen