Gene expression profiles in peripheral blood mononuclear cells of Asian obstructive sleep apnea patients

Biomed J. Mar-Apr 2014;37(2):60-70. doi: 10.4103/2319-4170.113188.

Abstract

Background: Obstructive sleep apnea (OSA) syndrome is a complicated disease involving several pathogenic mechanisms. Microarray technology provides a high-throughput means of identifying differentially expressed genes in patients with OSA.

Methods: An Affymetrix U133A gene chip was used to globally analyze the transcriptome of peripheral blood mononuclear cells (PBMC) in patients with OSA and nonapneic control participants after overnight polysomnography.

Results: Several genes were differentially expressed in patients with OSA compared with control participants. These genes included disintegrin and metalloproteinase domain 29 (ADAM29), solute carrier family 18 (vesicular acetylcholine) member 3 (SLC18A3), cyclin-dependent kinase inhibitor 2C (CDKN2C), and fibronectin-like domain-containing leucine-rich transmembrane protein 2 (FLRT2). Among these genes, the expression levels of ADAM29 (p = 0.00003), FLRT2 (p < 0.0001), and SLC18A3 (p = 0.0006) were found to be possible markers of severe OSA [respiratory disturbance index (RDI) > 30].

Conclusion: Our data suggest that PCMC expression of ADAM29, FLRT2, and SLC18A3 could be assessed as part of a routine screen to help identify individuals at risk of severe OSA in Asian populations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADAM Proteins / genetics*
  • Adult
  • Aged
  • Asians
  • Biomarkers / analysis
  • Female
  • Gene Expression Profiling
  • Humans
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / metabolism*
  • Male
  • Membrane Glycoproteins
  • Membrane Proteins / genetics*
  • Middle Aged
  • Risk Factors
  • Sleep Apnea, Obstructive / diagnosis
  • Sleep Apnea, Obstructive / genetics*
  • Transcriptome / genetics*
  • Vesicular Acetylcholine Transport Proteins / genetics*

Substances

  • Biomarkers
  • FLRT2 protein, human
  • Membrane Glycoproteins
  • Membrane Proteins
  • SLC18A3 protein, human
  • Vesicular Acetylcholine Transport Proteins
  • ADAM Proteins
  • ADAM29 protein, human