Abstract
Sensitive bioassay tissues for porcine endothelin-1 (ET-1) were developed in a cascade superfusion system and in organ baths. Venous preparations of the rabbit and rat were more sensitive than arterial preparations. ET-1 had a different pharmacological profile than Bay K 8644 on the various preparations. Nicardipine (0.1-1 microM) abolished the responses to Bay K 8644 without affecting those induced by ET-1. Thus, ET-1 contracts venous and some arterial vessels via specific receptors or channels that differ from dihydropyridine-sensitive calcium channels. Methylene blue and hemoglobin potentiated responses to ET-1 in endothelium-denuded venous vessels, whereas gossypol had no effect.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / pharmacology
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Animals
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Arteries / drug effects
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Calcium Channels / drug effects
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Calcium Channels / physiology*
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Endothelins
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Gossypol / pharmacology
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Hemoglobins / pharmacology
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In Vitro Techniques
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Indomethacin / pharmacology
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Male
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Methylene Blue / pharmacology
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Muscle Contraction / drug effects
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Muscle, Smooth, Vascular / drug effects*
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Nicardipine / pharmacology
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Peptides / antagonists & inhibitors*
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Peptides / pharmacology
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Rabbits
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Rats
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Rats, Inbred Strains
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Veins / drug effects
Substances
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Calcium Channels
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Endothelins
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Hemoglobins
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Peptides
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3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
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Nicardipine
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Gossypol
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Methylene Blue
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Indomethacin