Cellulomonas Pakistanensis Sp. Nov., a Moderately Halotolerant Actinobacteria

Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2305-2311. doi: 10.1099/ijs.0.059063-0. Epub 2014 Apr 14.

Abstract

A rod-shaped, motile, facultatively anaerobic and moderately halotolerant plant-growth-promoting actinobacterial strain, designated NCCP-11(T), was isolated from paddy grains. To delineate its taxonomic position, the strain was subjected to a polyphasic characterization. Cells of strain NCCP-11(T) grew at 10-37 °C (optimum 28-32 °C), at pH 6-9 (optimum pH 7) and in 0-12% (w/v) NaCl (optimum 1-2%) in broth medium. Based on 16S rRNA gene sequence analysis, strain NCCP-11(T) showed highest similarity to the type strains of Cellulomonas hominis (98.99%) and Cellulomonas denverensis (98.09 %) and less than 97 % with other closely related taxa. The chemotaxonomic data [major menaquinone: MK-9(H4); cell-wall peptidoglycan: type A4β; major fatty acids: anteiso-C15 : 0, C16 : 0, C14 : 0 and anteiso-C17 : 0; major polar lipids: diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannosides and two unknown polar lipids] also supported the affiliation of strain NCCP-11(T) to the genus Cellulomonas. The level of DNA-DNA relatedness between strain NCCP-11(T) and the two type strains mentioned above was less than 42.7%. On the basis of DNA-DNA relatedness, physiological and biochemical characteristics and phylogenetic position, strain NCCP-11(T) can be differentiated from species of the genus Cellulomonas with validly published names and thus represents a novel species, for which the name Cellulomonas pakistanensis sp. nov. is proposed. The type strain is NCCP-11(T) ( = DSM 24792(T) = JCM 18755(T) = KCTC 19798(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Cellulomonas / classification*
  • Cellulomonas / genetics
  • Cellulomonas / isolation & purification
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oryza / microbiology*
  • Pakistan
  • Peptidoglycan / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Seeds / microbiology
  • Sequence Analysis, DNA
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Peptidoglycan
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 9

Associated data

  • GENBANK/AB618146