Investigation of the peptide adsorption on ZrO2, TiZr, and TiO2 surfaces as a method for surface modification

ACS Appl Mater Interfaces. 2014 May 28;6(10):7408-16. doi: 10.1021/am500823m. Epub 2014 Apr 29.


Specific surface binding peptides offer a versatile and interesting possibility for the development of biocompatible implant materials. Therefore, eight peptide sequences were examined in regard to their adsorption on zirconium oxide (ZrO2), titanium zircon (TiZr), and titanium (c.p. Ti). Surface plasmon resonance (SPR) measurements were performed on Ti coated sensor chips to determine the kinetics of the interactions and kinetic rate constants (kon, koff, KD, and Rmax). We also investigated the interactions which are present in our system. Electrostatic and coordinative interactions were found to play a major role in the adsorption process. Four of the eight examined peptide sequences showed a significant adsorption on all investigated materials. Moreover, the two peptides with the highest adsorption could be quantified (up to 370 pmol/cm(2)). For potential biomaterials applications, we proved the stability of the adsorption of selected peptides in cell culture media, under competition with proteins and at body temperature (37 °C), and their biocompatibility via their effects on the adhesion and proliferation of human mesenchymal stem cells (hMSCs). The results qualify the peptides as anchor peptides for the biofunctionalization of implants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Amino Acid Sequence
  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / pharmacology
  • Cell Adhesion / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Humans
  • Hydrogen-Ion Concentration
  • Kinetics
  • Mesenchymal Stem Cells / cytology
  • Molecular Sequence Data
  • Osmolar Concentration
  • Peptides / chemistry*
  • Peptides / metabolism
  • Static Electricity
  • Surface Plasmon Resonance
  • Surface Properties
  • Titanium / chemistry*
  • Zirconium / chemistry*


  • Biocompatible Materials
  • Peptides
  • titanium dioxide
  • Zirconium
  • Titanium
  • zirconium oxide