24-hour rabbit heart storage with UW solution. Effects of low-flow perfusion, colloid, and shelf storage

Transplantation. 1989 Jul;48(1):6-9. doi: 10.1097/00007890-198907000-00002.

Abstract

A new technique for 24-hr cardiac preservation is described utilizing very low flow perfusion (microperfusion) with a cold flush solution. Rabbit hearts were arrested with UW solution and then perfused with the same solution through the aortic root at 0 degrees C at a rate of 3-6 ml/gm heart weight/24 hr. When tested on an ex vivo working heart model, the cardiac output (CO) was 28.72 +/- 7.69 ml/g/min compared with fresh UW flushed controls of 26.48 +/- 2.25 ml/g/min. Both oxygenated highflow perfusion with a more conventional perfusate and 24-hr ice storage with UW led to inferior results. Omission of the colloid, hydroxyethyl starch (HES), from the UW solution or prolonged shelf storage were also significantly detrimental. When a previously untested colloid, polyethylene glycol 20,000, was substituted for HES for microperfusion, excellent cardiac function was obtained. In fact, the mean CO of this group, 31.91 +/- 5.70, was significantly above that of fresh HES-UW unstored controls. The suggestion that the UW solution might be improved by this substitution warrants further study.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine
  • Allopurinol
  • Animals
  • Cardioplegic Solutions
  • Drug Stability
  • Glutathione
  • Heart*
  • Hydroxyethyl Starch Derivatives / pharmacology
  • Hypothermia, Induced
  • Insulin
  • Organ Preservation Solutions*
  • Organ Preservation*
  • Perfusion / methods
  • Rabbits
  • Raffinose
  • Solutions*
  • Time Factors

Substances

  • Cardioplegic Solutions
  • Hydroxyethyl Starch Derivatives
  • Insulin
  • Organ Preservation Solutions
  • Solutions
  • University of Wisconsin-lactobionate solution
  • Allopurinol
  • Glutathione
  • Adenosine
  • Raffinose