'Perforated Patch Recording' Allows Long-Term Monitoring of Metabolite-Induced Electrical Activity and Voltage-Dependent Ca2+ Currents in Pancreatic Islet B Cells

FEBS Lett. 1989 Jul 17;251(1-2):167-72. doi: 10.1016/0014-5793(89)81448-6.

Abstract

We describe the application of 'perforated patch recording' using the pore-forming antibiotic nystatin, to monitor the electrical activity and underlying ionic currents of rat and human pancreatic islet B cells. We demonstrate that glucose-induced electrical activity is seen even in single B cells during current-clamp recordings lasting hours 'L-type' Ca2+-channel currents can also be monitored over this period of time. This technique may prove useful in examining hormone and neurotransmitter modulation of electrical activity in B cells, while minimizing the effects of cytoplasmic 'wash-out'.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / pharmacology
  • Action Potentials / drug effects
  • Animals
  • Azides / pharmacology
  • Calcium Channels / physiology*
  • Diazoxide / pharmacology
  • Electric Conductivity
  • Glucose / pharmacology
  • Humans
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / physiology*
  • Membrane Potentials
  • Nifedipine / pharmacology
  • Rats
  • Sodium Azide
  • Tolbutamide / pharmacology

Substances

  • Azides
  • Calcium Channels
  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
  • Sodium Azide
  • Tolbutamide
  • Nifedipine
  • Glucose
  • Diazoxide