Clearance kinetics and tissue distribution of aggregated human serum IgA in rats

Immunology. 1989 Jun;67(2):274-80.


In the present study the clearance kinetics and tissue distribution of human polyclonal heat-aggregated serum IgA (AIgA) of different sizes in rats was studied after intravenous administration of 125I-AIgA. The 125I-AIgA of different sizes disappeared from the circulation in a biphasic manner with an initial rapid half-life (T1/2) and a second slower T1/2. The first T1/2 was related to the size of the 125I-AIgA: high molecular weight (MW) 125I-AIgA was cleared much faster than 125I-AIgA with a low MW. Relatively more degradation products were observed in blood when high MW 125I-AIgA were injected as compared to low MW 125I-AIgA. The AIgA were mainly taken up by the liver. Eight minutes after injection of high MW 125I-AIgA, 90% of the injected dose was found in the liver, whereas less than 2% was detected in the spleen. Very little activity was detectable in other organs, such as lungs, heart and kidneys. In the present study 1-3% of the injected 125I-AIgA were found in the bile. Analysis of this material revealed that low MW 125I-AIgA were transported more efficiently to the bile than high MW 125I-AIgA. To obtain more insight into the receptors involved in the clearance of 125I-AIgA, rats were pretreated with ovalbumin or asialofetuin. The clearance of 125I-AIgA of different sizes was inhibited when rats were pretreated with asialofetuin. Pretreatment with ovalbumin had no effect on the clearance rates of 125I-AIgA. These results suggest a role for carbohydrate receptors, which recognize glycoprotein-containing galactose terminal residues on Kupffer cells, in the clearance of 125I-AIgA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Asialoglycoproteins*
  • Binding, Competitive
  • Fetuins
  • Humans
  • Immunoglobulin A / pharmacokinetics*
  • Injections, Intravenous
  • Liver / metabolism
  • Macromolecular Substances
  • Male
  • Metabolic Clearance Rate / drug effects
  • Molecular Weight
  • Ovalbumin / administration & dosage
  • Rats
  • Rats, Inbred Strains
  • Sodium Chloride / administration & dosage
  • Tissue Distribution
  • alpha-Fetoproteins / administration & dosage


  • Asialoglycoproteins
  • Fetuins
  • Immunoglobulin A
  • Macromolecular Substances
  • alpha-Fetoproteins
  • asialofetuin
  • Sodium Chloride
  • Ovalbumin