The present investigation was undertaken to examine the organization of peptidergic projections that exist between the parabrachial nuclear complex (PB) and the central nucleus of the amygdala (CNA). The retrograde tracer True Blue was injected into the CNA of adult rats. The brain tissue was then reacted immunocytochemically to localize neurotensin (NT), substance P (SP), methionine enkephalin (ENK), vasoactive intestinal polypeptide (VIP), somatostatin (SS), and cholecystokinin octapeptide (CCK). Following microinjection of True Blue in the CNA, retrogradely-labeled neurons were located primarily in the external lateral subnucleus, abutting the brachium conjunctivum. In animals that received colchicine pretreatment, two populations of neurons, containing either SP or NT, were found to project to the CNA. In addition, cells containing CCK, ENK, VIP, or SS were not found to be a part of this projection system. These data suggest that neurons in the PB project to the CNA and are, in part peptide-containing.