The effect of sildenafil and udenafil on testicular damage following ischemia-reperfusion injury in rats

J Urol. 2014 Oct;192(4):1272-7. doi: 10.1016/j.juro.2014.04.011. Epub 2014 Apr 15.


Purpose: Ischemia-reperfusion injury can cause testicular damage and phosphodiesterase inhibitors are reported to regulate antioxidant activity. We investigated the prevention of ipsilateral and contralateral testicular damage using 2 phosphodiesterase inhibitors after testicular detorsion in rats.

Materials and methods: A total of 28 adult male rats were randomly divided into 4 groups of 7 each, including group 1-sham operation, group 2-testicular torsion and detorsion, group 3- testicular torsion and detorsion with sildenafil administration before detorsion and group 4- testicular torsion and detorsion with udenafil administration before detorsion. Tissue levels of malondialdehyde, total sulfhydryl and nitrite were evaluated, and histopathological changes in the groups were examined.

Results: Compared to group 1 significantly increased tissue malondialdehyde (p = 0.001), significantly decreased total sulfhydryl (p = 0.038) and insignificantly increased nitrite were found in group 2. Compared to group 2 malondialdehyde decreased significantly and total sulfhydryl increased significantly in groups 3 and 4. The decrease in nitrite was insignificant in the latter 2 groups. Histopathology revealed increased hemorrhage, congestion and edema in group 2 rats. The testicular injury score was lower in groups 3 and 4. In group 2 grades II to IV injury was detected while most specimens in treated groups showed grade II injury.

Conclusions: This study indicates that intraperitoneal administration of sildenafil and udenafil efficiently suppresses radical production while decreasing histological changes after testicular ischemia-reperfusion injury.

Keywords: reperfusion injury; sildenafil; spermatic cord torsion; testis; udenafil.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Disease Models, Animal
  • Drug Therapy, Combination
  • Injections, Intraperitoneal
  • Male
  • Phosphodiesterase 5 Inhibitors / administration & dosage
  • Piperazines / administration & dosage*
  • Purines / administration & dosage
  • Pyrimidines / administration & dosage*
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / complications*
  • Reperfusion Injury / pathology
  • Sildenafil Citrate
  • Sulfonamides / administration & dosage*
  • Sulfones / administration & dosage*
  • Testicular Diseases / drug therapy*
  • Testicular Diseases / metabolism
  • Testicular Diseases / pathology
  • Testis / drug effects*
  • Testis / pathology


  • Phosphodiesterase 5 Inhibitors
  • Piperazines
  • Purines
  • Pyrimidines
  • Sulfonamides
  • Sulfones
  • Sildenafil Citrate
  • udenafil