Zona pellucida-induced acrosome reaction in boar sperm

Biol Reprod. 1989 Mar;40(3):525-30. doi: 10.1095/biolreprod40.3.525.

Abstract

Induction of the acrosome reaction in boar sperm by the zona pellucida (ZP) was investigated. A modified cytochemical staining method for measuring the acrosome reaction in boar sperm gave equivalent results to those obtained with transmission electron microscopy. Isolated heat-solubilized ZP effectively induced the acrosome reaction in boar sperm at a concentration of 25 micrograms/ml. Electrophoretically purified ZP components were also tested for acrosome reaction-inducing activity; both the 55,000 and 90,000 components of the ZP were effective. The carbohydrate moiety of the 55,000 component was necessary for activity because the polypeptides derived by chemical deglycosylation of the two glycoproteins did not induce the acrosome reaction.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acrosome / cytology*
  • Animals
  • Female
  • Glycoproteins / pharmacology
  • Male
  • Microscopy, Electron
  • Ovum / physiology*
  • Peptides / pharmacology
  • Sperm Motility
  • Sperm-Ovum Interactions*
  • Spermatozoa / analysis
  • Spermatozoa / cytology*
  • Spermatozoa / metabolism*
  • Staining and Labeling
  • Swine
  • Zona Pellucida / physiology*

Substances

  • Glycoproteins
  • Peptides