The effect of high-fat--high-fructose diet on skeletal muscle mitochondrial energetics in adult rats

Eur J Nutr. 2015 Mar;54(2):183-92. doi: 10.1007/s00394-014-0699-7. Epub 2014 Apr 18.


Purpose: To study the effect of isoenergetic administration to adult rats of high-fat or high-fat--high-fructose diet for 2 weeks on skeletal muscle mitochondrial energetic.

Methods: Body and skeletal muscle composition, energy balance, plasma lipid profile and glucose tolerance were measured, together with mitochondrial functionality, oxidative stress and antioxidant defense.

Results: Rats fed high-fat--high-fructose diet exhibited significantly higher plasma triglycerides and non-esterified fatty acids, together with significantly higher plasma glucose and insulin response to glucose load. Skeletal muscle triglycerides and ceramide were significantly higher in rats fed high-fat--high-fructose diet. Skeletal muscle mitochondrial energetic efficiency and uncoupling protein 3 content were significantly higher, while adenine nucleotide translocase content was significantly lower, in rats fed high-fat or high-fat--high-fructose diet.

Conclusions: The results suggest that a high-fat--high-fructose diet even without hyperphagia is able to increase lipid flow to skeletal muscle and mitochondrial energetic efficiency, with two detrimental effects: (a) energy sparing that contributes to the early onset of obesity and (b) reduced oxidation of fatty acids and lipid accumulation in skeletal muscle, which could generate insulin resistance.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ceramides / metabolism
  • Diet, High-Fat / adverse effects*
  • Dietary Carbohydrates / adverse effects*
  • Energy Intake
  • Energy Metabolism*
  • Fatty Acids, Nonesterified / blood
  • Fructose / adverse effects*
  • Hindlimb
  • Hyperlipidemias / blood
  • Hyperlipidemias / etiology
  • Hyperlipidemias / metabolism
  • Insulin Resistance*
  • Ion Channels / metabolism
  • Male
  • Mitochondria, Muscle / enzymology
  • Mitochondria, Muscle / metabolism*
  • Mitochondrial ADP, ATP Translocases / metabolism
  • Mitochondrial Proteins / metabolism
  • Muscle, Skeletal / enzymology
  • Muscle, Skeletal / metabolism*
  • Oxidative Phosphorylation
  • Oxidative Stress
  • Rats, Sprague-Dawley
  • Triglycerides / blood
  • Triglycerides / metabolism
  • Uncoupling Protein 3
  • Weight Gain


  • Ceramides
  • Dietary Carbohydrates
  • Fatty Acids, Nonesterified
  • Ion Channels
  • Mitochondrial Proteins
  • Triglycerides
  • Ucp3 protein, rat
  • Uncoupling Protein 3
  • Fructose
  • Mitochondrial ADP, ATP Translocases