Evolutionary engineering of yeast

Methods Mol Biol. 2014;1152:169-83. doi: 10.1007/978-1-4939-0563-8_10.


Evolutionary engineering is an inverse metabolic engineering strategy which is based on increasing genetic diversity and screening large populations for desired phenotypes. This strategy is highly advantageous in certain situations over rational metabolic engineering approaches, since there is little or no requirement of detailed genetic background information for the trait of interest. Here, we describe the experimental methodology for selecting stress-resistant yeast strains via evolutionary engineering approach by either serial batch or chemostat cultivations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Evolution, Molecular*
  • Genetic Engineering / methods*
  • Mutagenesis
  • Mutation
  • Phenotype
  • Saccharomyces cerevisiae / genetics*