The films organized with biomolecules and organic materials are important elements for developing bioelectronic devices according to their electron transfer property. Until now, several concepts of techniques have been accomplished to be used for developing biomemory devices. However it is difficult to detect the current signal from the electron transfer between biomolecules and the substrate in these fabricated films. To enhance the current signal, the silver nanoparticle was introduced to the cytochrome c in this present study. The surface morphology of the fabricated film was investigated by atomic force microscopy. The current signal enhancement was investigated by cyclic voltammetry. As a result, we could obtain the redox potentials. Moreover, by chronoamperometry, we validated that this proposed layer showed the signal-enhanced memory property for biomemory devices. This new film composed of the cytochrome c and the silver nanoparticle showed the signal enhancement. Using chronoamperometry, the areas under the graphs between 0 s and 50 ms were calculated. The calculated result showed that the areas under the cytochrome c/SNP graph and cytochrome c graph were 6.93 x 10(-7) C and 4.54 x 10(-7) C, respectively. This numerical value verified that the cytochrome c/silver nanoparticle hetero-layer film showed better electron charged biomemory performance compared to the cytochrome c monolayer. This signal-enhanced film can be applied to the bioelectronic devices which are able to replace existing electronic devices in the near future.