[Effects of low-frequency electrical stimulation of hippocampus on the expression of GABAA receptor α1 and β2 subunits in kainate-kindled rats]

Zhonghua Yi Xue Za Zhi. 2014 Feb 11;94(5):382-5.
[Article in Chinese]

Abstract

Objective: To explore the effects of low-frequency electrical stimulation (LFS) of hippocampus on the expression of GABAA receptor α1 and β2 subunits in the kainite (KA)-kindled rats and explore its underlying rescue mechanism for temporal lobe epilepsy.

Methods: The SD rats were divided into 4 groups (n = 10):control group:no operation; epilepsy group:KA was stereotactically injected into rat hippocampus to establish KA-kindled model; sham-LFS group:only implanting of electrodes into hippocampus without LFS; LFS group:KA-kindled model with LFS. Then hippocampal tissues were collected and their expressions of GABAA receptor α1 and β2 subunits measured by immunohistochemistry, quantitative-polymerase chain reaction (q-PCR) and Western blot.Nissl staining was performed to detect the number of damaged hippocampal neurons.

Results: There were significant decreases of GABAA receptor α1 and β2 subunits in kindled hippocampus (P < 0.01). LFS resulted in an effective rescue, but failed to reach normal levels. The damaged neurons could not be reversed according to Nissl staining.

Conclusion: The hippocampal application of LFS may protect against seizures by modulating the expressions of α1 and β2 GABAA receptor subunits.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Electric Stimulation
  • Epilepsy / metabolism
  • Hippocampus / metabolism*
  • Kainic Acid / adverse effects
  • Kindling, Neurologic
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, GABA-A / metabolism*
  • Synapses / metabolism*

Substances

  • Receptors, GABA-A
  • Kainic Acid