Soni-removal of nucleic acids from inclusion bodies

Biochem Biophys Res Commun. 2014 May 23;448(1):45-9. doi: 10.1016/j.bbrc.2014.04.049. Epub 2014 Apr 18.


Inclusion bodies (IBs) are commonly formed in Escherichia coli due to over expression of recombinant proteins in non-native state. Isolation, denaturation and refolding of these IBs is generally performed to obtain functional protein. However, during this process IBs tend to form non-specific interactions with sheared nucleic acids from the genome, thus getting carried over into downstream processes. This may hinder the refolding of IBs into their native state. To circumvent this, we demonstrate a methodology termed soni-removal which involves disruption of nucleic acid-inclusion body interaction using sonication; followed by solvent based separation. As opposed to conventional techniques that use enzymes and column-based separations, soni-removal is a cost effective alternative for complete elimination of buried and/or strongly bound short nucleic acid contaminants from IBs.

Keywords: Economic; Inclusion bodies purification; Nucleic acid contamination; Soni-Removal; Sonication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Fractionation / methods*
  • Dengue Virus / chemistry
  • Hyaluronan Receptors / isolation & purification
  • Inclusion Bodies / metabolism*
  • Nucleic Acids / isolation & purification*
  • Nucleic Acids / metabolism
  • Protein Denaturation
  • Protein Folding
  • Recombinant Proteins / isolation & purification
  • Solubility
  • Sonication
  • Viral Envelope Proteins / isolation & purification


  • Hyaluronan Receptors
  • Nucleic Acids
  • Recombinant Proteins
  • Viral Envelope Proteins