Effects of gypenosides on anxiety disorders in MPTP-lesioned mouse model of Parkinson's disease

Brain Res. 2014 Jun 3;1567:57-65. doi: 10.1016/j.brainres.2014.04.015. Epub 2014 Apr 18.

Abstract

Ethanol extract (GP-EX) of Gynostemma pentaphyllum (GP) ameliorates chronic stress-induced anxiety in mice. The present study investigated the effects of gypenoside-enriched components (GPS), GP-EX and water extract of GP (GP-WX) on MPTP lesion-induced affective disorders in C57BL/6 mice. GPS (50mg/kg) and GP-EX (50mg/kg) for 21 day-treatment period improved the symptom of anxiety disorders in the MPTP-lesioned mouse model of PD with or without L-DOPA treatment, which was examined by the elevated plus-maze and marble burying tests. In these states, treatments with GPS (50mg/kg) and GP-EX (50mg/kg) significantly increased the brain levels of dopamine and serotonin in the MPTP-lesioned mouse model of PD with or without l-DOPA treatment. In addition, treatments with GPS (50mg/kg) and GP-EX (50mg/kg) showed protective effects on dopaminergic neurons in MPTP-lesioned mouse model of PD with or without L-DOPA treatment. In contrast, GPS (30 mg/kg) and GP-WX (50mg/kg) showed anxiolytic effects in the same animal models, but it was not significant. These results suggest that GPS (50mg/kg) and GP-EX (50mg/kg) showed anxiolytic effects on affective disorders and protective effects on dopaminergic neurons by modulating the brain levels of dopamine and serotonin in the MPTP-lesioned mouse model of PD with or without l-DOPA treatment. Clinical trials of GPS and GP-EX need to be conducted further so as to develop adjuvant therapeutic agents for PD patients.

Keywords: Dopamine; Elevated plus-maze; Gynostemma pentaphyllum; Gypenosides; MPTP; Serotonin.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Anxiety Agents / pharmacology*
  • Antiparkinson Agents / pharmacology
  • Anxiety Disorders / drug therapy*
  • Anxiety Disorders / etiology*
  • Anxiety Disorders / pathology
  • Anxiety Disorders / physiopathology
  • Behavior, Animal / drug effects
  • Brain / drug effects
  • Brain / pathology
  • Brain / physiopathology
  • Dopamine / metabolism
  • Dopaminergic Neurons / drug effects
  • Dopaminergic Neurons / pathology
  • Dopaminergic Neurons / physiology
  • Ethanol / chemistry
  • Gynostemma*
  • Levodopa / pharmacology
  • Male
  • Maze Learning / drug effects
  • Mice, Inbred C57BL
  • Neuroprotective Agents / pharmacology
  • Neuropsychological Tests
  • Parkinsonian Disorders / complications*
  • Parkinsonian Disorders / drug therapy*
  • Parkinsonian Disorders / pathology
  • Parkinsonian Disorders / physiopathology
  • Phytotherapy
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Serotonin / metabolism
  • Water / chemistry

Substances

  • Anti-Anxiety Agents
  • Antiparkinson Agents
  • Neuroprotective Agents
  • Plant Extracts
  • gypenoside
  • Water
  • Serotonin
  • Ethanol
  • Levodopa
  • Dopamine