Targeting c-Met receptor overcomes TRAIL-resistance in brain tumors

PLoS One. 2014 Apr 18;9(4):e95490. doi: 10.1371/journal.pone.0095490. eCollection 2014.

Abstract

Tumor necrosis factor related apoptosis-inducing ligand (TRAIL) induced apoptosis specifically in tumor cells. However, with approximately half of all known tumor lines being resistant to TRAIL, the identification of TRAIL sensitizers and their mechanism of action become critical to broadly use TRAIL as a therapeutic agent. In this study, we explored whether c-Met protein contributes to TRAIL sensitivity. We found a direct correlation between the c-Met expression level and TRAIL resistance. We show that the knock down c-Met protein, but not inhibition, sensitized brain tumor cells to TRAIL-mediated apoptosis by interrupting the interaction between c-Met and TRAIL cognate death receptor (DR) 5. This interruption greatly induces the formation of death-inducing signaling complex (DISC) and subsequent downstream apoptosis signaling. Using intracranially implanted brain tumor cells and stem cell (SC) lines engineered with different combinations of fluorescent and bioluminescent proteins, we show that SC expressing a potent and secretable TRAIL (S-TRAIL) have a significant anti-tumor effect in mice bearing c-Met knock down of TRAIL-resistant brain tumors. To our best knowledge, this is the first study that demonstrates c-Met contributes to TRAIL sensitivity of brain tumor cells and has implications for developing effective therapies for brain tumor patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Apoptosis / genetics
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / metabolism
  • Cell Line, Tumor
  • Death Domain Receptor Signaling Adaptor Proteins / metabolism
  • Drug Resistance, Neoplasm / genetics*
  • Gene Knockdown Techniques
  • Humans
  • Medulloblastoma / genetics
  • Medulloblastoma / metabolism
  • Mice
  • Protein Binding
  • Proto-Oncogene Proteins c-met / genetics*
  • Proto-Oncogene Proteins c-met / metabolism
  • Receptors, TNF-Related Apoptosis-Inducing Ligand / metabolism
  • Stem Cells / metabolism
  • TNF-Related Apoptosis-Inducing Ligand / genetics*
  • TNF-Related Apoptosis-Inducing Ligand / metabolism
  • TNF-Related Apoptosis-Inducing Ligand / pharmacology

Substances

  • Death Domain Receptor Signaling Adaptor Proteins
  • Receptors, TNF-Related Apoptosis-Inducing Ligand
  • TNF-Related Apoptosis-Inducing Ligand
  • Proto-Oncogene Proteins c-met

Grant support

This work was supported by the James McDonald Foundation(KS),grant # 220020245. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.