Involvement of satellite I noncoding RNA in regulation of chromosome segregation

Genes Cells. 2014 Jun;19(6):528-38. doi: 10.1111/gtc.12149. Epub 2014 Apr 21.

Abstract

Human centromeres consist of repetitive sequences from which satellite I noncoding RNAs are transcribed. We found that knockdown of satellite I RNA causes abnormal chromosome segregation and generation of nuclei with a grape-shape phenotype. Co-immunoprecipitation experiments showed that satellite I RNA associates with Aurora B, a component of the chromosome passenger complex (CPC) regulating proper attachment of microtubules to kinetochores, in mitotic HeLa cells. Satellite I RNA was also shown to associate with INCENP, another component of the CPC. In addition, depletion of satellite I RNA resulted in up-regulation of kinase activity of Aurora B and delocalization of the CPC from the centromere region. These results suggest that satellite I RNA is involved in chromosome segregation through controlling activity and centromeric localization of Aurora B kinase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aurora Kinase B / genetics
  • Aurora Kinase B / metabolism
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism
  • Cell Nucleus Division
  • Centromere / genetics
  • Centromere / metabolism
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism
  • Chromosome Segregation / physiology*
  • HeLa Cells
  • Humans
  • Interphase
  • RNA, Untranslated / metabolism*

Substances

  • Chromosomal Proteins, Non-Histone
  • INCENP protein, human
  • RNA, Untranslated
  • AURKB protein, human
  • Aurora Kinase B