Abstract
The crude polysaccharides (GFP) were isolated from the fruiting bodies of Grifola frondosa and purified by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography in that order. Three main fractions, GFP-1, GFP-2 and GFP-3, were obtained through the isolation and purification steps. Then the antioxidant activities of these three fractions were investigated in vitro. The results showed that GFP-1, GFP-2 and GFP-3 possessed significant inhibitory effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical; their reducing power, ferrous ions chelating effect and the inhibition ability of the rat liver lipid oxidation where also strong. These results suggest that G. frondosa polysaccharides could be a suitable natural antioxidant and may be the functional foods for humans.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants* / chemistry
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Antioxidants* / isolation & purification
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Antioxidants* / pharmacology
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Biphenyl Compounds / chemistry
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Chelating Agents* / chemistry
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Chelating Agents* / isolation & purification
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Chelating Agents* / pharmacology
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Ferricyanides / chemistry
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Ferrous Compounds / chemistry
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Fruiting Bodies, Fungal
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Fungal Polysaccharides* / chemistry
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Fungal Polysaccharides* / isolation & purification
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Fungal Polysaccharides* / pharmacology
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Grifola*
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Hydroxyl Radical / chemistry
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Lipid Peroxidation / drug effects
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Liver / drug effects
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Liver / metabolism
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Male
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Oxidation-Reduction
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Picrates / chemistry
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Rats, Sprague-Dawley
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Superoxides / chemistry
Substances
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Antioxidants
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Biphenyl Compounds
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Chelating Agents
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Ferricyanides
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Ferrous Compounds
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Fungal Polysaccharides
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Picrates
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Superoxides
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Hydroxyl Radical
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1,1-diphenyl-2-picrylhydrazyl
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potassium ferricyanide