Purification of a polysaccharide from Gynostemma pentaphyllum Makino and its therapeutic advantages for psoriasis

Carbohydr Polym. 2012 Aug 1;89(4):1232-7. doi: 10.1016/j.carbpol.2012.04.001. Epub 2012 Apr 7.


In current study, a water-soluble polysaccharide (GP-I), with a molecular mass of 33 kDa, was purified from Gynostemma pentaphyllum. Gas chromatography (GC) analysis suggested that it was composed of Glc, Gal, Man, Rha and Ara with a ratio of 5.3: 4.2: 3.0: 0.7: 0.8. The GP-I (25, 50, 100, 200 and 400 μg/ml) was found to have significant anti-proliferative effects on HaCat cells in a dose-dependent manner, as measured by MTT assay. On the contrary, Trypan blue exclusion experiment indicated that GP-I had no cytotoxicity to HaCat cells. Moreover, the decrease of mitochondrial membrane potential (MMP) in GP-I treated cells was also observed, indicating apoptosis in HaCat cells. Besides, tumor necrosis factor-α (TNF-α), a vital pro-inflammatory cytokine in psoriasis, in the supernatant of HaCat cells was dramatically reduced by GP-I. Collectively, these findings suggested that GP-I was a promising agent to be developed for psoriasis treatment in clinical therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects*
  • Cell Line
  • Dose-Response Relationship, Drug
  • Gynostemma / chemistry*
  • Humans
  • Membrane Potential, Mitochondrial / drug effects*
  • Polysaccharides* / chemistry
  • Polysaccharides* / isolation & purification
  • Polysaccharides* / pharmacology
  • Psoriasis* / drug therapy
  • Psoriasis* / metabolism
  • Psoriasis* / pathology
  • Tumor Necrosis Factor-alpha / metabolism*


  • Polysaccharides
  • Tumor Necrosis Factor-alpha