Convergence of serotonin-, enkephalin- and substance P-like immunoreactive afferent fibers onto cat medullary dorsal horn projection neurons: a triple immunocytochemical staining technique combined with the retrograde HRP-tracing method

Brain Res. 1989 Jul 10;491(2):360-5. doi: 10.1016/0006-8993(89)90071-1.

Abstract

Convergence of serotonin (5-HT)-, enkephalin (ENK)-, and substance P (SP)-like immunoreactive (LI) afferent fibers onto cat medullary dorsal horn projection neurons was examined by retrogradely labeling trigeminothalamic neurons and immunocytochemically labeling 5-HT, ENK and SP fibers in the same tissue section using distinct chromogens. Convergence of 5-HT-, ENK- and SP-LI fibers upon single projection neurons was often observed in laminae I and V.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cats
  • Enkephalins / metabolism*
  • Horseradish Peroxidase
  • Immunohistochemistry
  • Neurons, Afferent / cytology
  • Neurons, Afferent / metabolism*
  • Serotonin / metabolism*
  • Spinal Cord / cytology
  • Spinal Cord / metabolism*
  • Substance P / metabolism*

Substances

  • Enkephalins
  • Serotonin
  • Substance P
  • Horseradish Peroxidase