Abstract
An isatoic anhydride derivative conjugated to a biotin and a disulfide linker was specifically designed for the separation of nucleic acids. Starting from a DNA-RNA mixture, a selective 2'-hydroxyl acylation of RNAs followed by capture with streptavidin-coated magnetic beads and cleavage of the disulfide led to elution of RNAs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acylation
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Anhydrides / chemistry*
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Biotin / chemistry*
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DNA / chemistry*
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DNA / isolation & purification*
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Hydroxides / chemistry*
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RNA / chemistry*
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RNA / isolation & purification*
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RNA, Viral / chemistry
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RNA, Viral / isolation & purification
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Streptavidin / chemistry
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ortho-Aminobenzoates / chemistry*
Substances
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Anhydrides
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Hydroxides
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RNA, Viral
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ortho-Aminobenzoates
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N-methylisatoic anhydride
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RNA
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Biotin
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DNA
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Streptavidin
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hydroxide ion