Biotin-conjugated N-methylisatoic anhydride: a chemical tool for nucleic acid separation by selective 2'-hydroxyl acylation of RNA

Chem Commun (Camb). 2014 Jun 1;50(43):5748-51. doi: 10.1039/c4cc01134a. Epub 2014 Apr 22.

Abstract

An isatoic anhydride derivative conjugated to a biotin and a disulfide linker was specifically designed for the separation of nucleic acids. Starting from a DNA-RNA mixture, a selective 2'-hydroxyl acylation of RNAs followed by capture with streptavidin-coated magnetic beads and cleavage of the disulfide led to elution of RNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acylation
  • Anhydrides / chemistry*
  • Biotin / chemistry*
  • DNA / chemistry*
  • DNA / isolation & purification*
  • Hydroxides / chemistry*
  • RNA / chemistry*
  • RNA / isolation & purification*
  • RNA, Viral / chemistry
  • RNA, Viral / isolation & purification
  • Streptavidin / chemistry
  • ortho-Aminobenzoates / chemistry*

Substances

  • Anhydrides
  • Hydroxides
  • RNA, Viral
  • ortho-Aminobenzoates
  • N-methylisatoic anhydride
  • RNA
  • Biotin
  • DNA
  • Streptavidin
  • hydroxide ion