Vitamin D supplementation enhances the fixation of titanium implants in chronic kidney disease mice

PLoS One. 2014 Apr 21;9(4):e95689. doi: 10.1371/journal.pone.0095689. eCollection 2014.


Vitamin D (Vit D) deficiency is a common condition in chronic kidney disease (CKD) patients that negatively affects bone regeneration and fracture healing. Previous study has shown that timely healing of titanium implants is impaired in CKD. This study aimed to investigate the effect of Vit D supplementation on implant osseointegration in CKD mice. Uremia was induced by 5/6 nephrectomy in C57BL mice. Eight weeks after the second renal surgery, animals were given 1,25(OH)2D3 three times a week intraperitoneally for four weeks. Experimental titanium implants were inserted into the distal end of femurs two weeks later. Serum measurements confirmed decreased 1,25(OH)2D levels in CKD mice, which could be successfully corrected by Vit D injections. Moreover, the hyperparathyroidism observed in CKD mice was also corrected. X-ray examination and histological sections showed successful osseointegration in these mice. Histomorphometrical analysis revealed that the bone-implant contact (BIC) ratio and bone volume (BV/TV) around the implant were significantly increased in the Vit D-supplementation group. In addition, resistance of the implant, as measured by a push-in method, was significantly improved compared to that in the vehicle group. These results demonstrate that Vit D supplementation is an effective approach to improve the fixation of titanium implants in CKD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dietary Supplements*
  • Female
  • Femur / diagnostic imaging
  • Femur / drug effects
  • Implants, Experimental*
  • Materials Testing
  • Mice, Inbred C57BL
  • Orthopedic Fixation Devices*
  • Radiography
  • Renal Insufficiency, Chronic / blood
  • Renal Insufficiency, Chronic / diagnostic imaging
  • Renal Insufficiency, Chronic / therapy*
  • Titanium / pharmacology*
  • Vitamin D / pharmacology*
  • Wound Healing / drug effects


  • Vitamin D
  • Titanium

Grant support

This work was supported by grants from the National Natural Science Foundation of China (NSFC 81371173), New Century Excellent Talents in University (NCET-12-0379) and International Team of Implantology (ITI 717_2010) to Q.Y., and the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK, R01-073944) to B.L. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.