A nondigestible saccharide, fructooligosaccharide, increases the promotive effect of a flavonoid, α-glucosyl-isoquercitrin, on glucagon-like peptide 1 (GLP-1) secretion in rat intestine and enteroendocrine cells

Mol Nutr Food Res. 2014 Jul;58(7):1581-4. doi: 10.1002/mnfr.201300871. Epub 2014 Apr 22.


This study conducted in vivo and in situ experiments with rats to investigate the glucagon-like peptide-1 (GLP-1) secretion in response to oral or ileal administration of α-glucosyl-isoquercitrin (20-40 mmol in 2 mL; Q3G), fructooligosaccharides (200 mmol in 2 mL; FOS) and Q3G + FOS. Direct effects on GLP-1-producing l-cells were also examined by an in vitro study using a murine enteroendocrine cell line, GLUTag. To evaluate the plasma GLP-1 level, blood samples from jugular cannula for in vivo and portal cannula for in situ experiments were obtained before and after administration of Q3G, FOS, or Q3G + FOS. We found tendencies for increases but transient stimulation of GLP-1 secretion by Q3G in in vivo and in situ experiments. Although FOS alone did not have any effects, Q3G + FOS enhanced and prolonged high plasma GLP-1 level in both experiments. In addition, application of Q3G on GLUTag cells stimulated GLP-1 secretion while FOS enhanced the effect of Q3G. Our results suggest that Q3G + FOS possess the potential for the management or prevention of Type 2 diabetes mellitus (T2DM) by enhancing and prolonging the GLP-1 secretion via direct stimulation of GLP-1 producing l-cell.

Keywords: Fructooligosaccharides; Glucagon-like peptide-1; α-Glucosyl-isoquercitrin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Diabetes Mellitus, Type 2 / prevention & control
  • Enteroendocrine Cells / drug effects*
  • Enteroendocrine Cells / metabolism
  • Glucagon-Like Peptide 1 / metabolism*
  • Ileum / cytology
  • Ileum / drug effects*
  • Ileum / metabolism
  • Male
  • Oligosaccharides / pharmacology*
  • Quercetin / analogs & derivatives*
  • Quercetin / pharmacology
  • Rats
  • Rats, Wistar


  • Oligosaccharides
  • fructooligosaccharide
  • isoquercitrin
  • Glucagon-Like Peptide 1
  • Quercetin