Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

S Baghel; Y K Bansal

doi:10.1007/s12298-014-0229-3

Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

Physiol Mol Biol Plants. 2014 Apr;20(2):241-7. doi: 10.1007/s12298-014-0229-3. Epub 2014 Apr 3.

Authors

S Baghel¹, Y K Bansal¹

Affiliation

¹ Plant Tissue Culture Laboratory, Department of Biological Science, R. D. University, Jabalpur, India.

Abstract

Apical and axillary buds of Guizotia abyssinica Cass., isolated from seedlings raised in vitro, were cultured. High frequency of shoot regeneration was achieved on MS medium with BAP (1 mgl(-1)). Effect of BAP, Kn and GA3 applied successively in culture on shoot regeneration and flower bud formation has been studied. The shoots differentiated in cultures elongated on this medium. These rooted subsequently on half strength MS medium. The shoots flowered in vitro on MS medium with a combination of BAP (0.1mgl(-1)) + GA3 (0.1 mgl(-1)). The plantlets thus formed were successfully hardened with 90 % survival.

Keywords: Biodiesel; Guizotia abyssinica; In vitro flowering; Niger; Plant growth regulators.