Changes in subcutaneous fat cell volume and insulin sensitivity after weight loss

Diabetes Care. 2014 Jul;37(7):1831-6. doi: 10.2337/dc13-2395. Epub 2014 Apr 23.

Abstract

Objective: Large subcutaneous fat cells associate with insulin resistance and high risk of developing type 2 diabetes. We investigated if changes in fat cell volume and fat mass correlate with improvements in the metabolic risk profile after bariatric surgery in obese patients.

Research design and methods: Fat cell volume and number were measured in abdominal subcutaneous adipose tissue in 62 obese women before and 2 years after Roux-en-Y gastric bypass (RYGB). Regional body fat mass by dual-energy X-ray absorptiometry; insulin sensitivity by hyperinsulinemic-euglycemic clamp; and plasma glucose, insulin, and lipid profile were assessed.

Results: RYGB decreased body weight by 33%, which was accompanied by decreased adipocyte volume but not number. Fat mass in the measured regions decreased and all metabolic parameters were improved after RYGB (P < 0.0001). Whereas reduced subcutaneous fat cell size correlated strongly with improved insulin sensitivity (P = 0.0057), regional changes in fat mass did not, except for a weak correlation between changes in visceral fat mass and insulin sensitivity and triglycerides. The curve-linear relationship between fat cell size and fat mass was altered after weight loss (P = 0.03).

Conclusions: After bariatric surgery in obese women, a reduction in subcutaneous fat cell volume associates more strongly with improvement of insulin sensitivity than fat mass reduction per se. An altered relationship between adipocyte size and fat mass may be important for improving insulin sensitivity after weight loss. Fat cell size reduction could constitute a target to improve insulin sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorptiometry, Photon
  • Adult
  • Blood Glucose / metabolism
  • Cell Size
  • Female
  • Gastric Bypass / methods*
  • Glucose Clamp Technique
  • Humans
  • Insulin / blood
  • Insulin Resistance*
  • Intra-Abdominal Fat / metabolism
  • Lipids / blood
  • Middle Aged
  • Obesity / surgery*
  • Prospective Studies
  • Subcutaneous Fat / cytology*
  • Triglycerides / blood
  • Triglycerides / physiology
  • Weight Loss / physiology

Substances

  • Blood Glucose
  • Insulin
  • Lipids
  • Triglycerides