Exon 11 skipping of SCN10A coding for voltage-gated sodium channels in dorsal root ganglia

Channels (Austin). 2014;8(3):210-5. doi: 10.4161/chan.28146.


The voltage-gated sodium channel Na(V)1.8 (encoded by SCN10A) is predominantly expressed in dorsal root ganglia(DRG) and plays a critical role in pain perception. We analyzed SCN10A transcripts isolated from human DRGs using deep sequencing and found a novel splice variant lacking exon 11, which codes for 98 amino acids of the domain I/II linker. Quantitative PCR analysis revealed an abundance of this variant of up to 5–10% in human, while no such variants were detected in mouse or rat. Since no obvious functional differences between channels with and without the exon-11 sequence were detected, it is suggested that SCN10A exon 11 skipping in humans is a tolerated event.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • Exons*
  • Ganglia, Spinal / metabolism*
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • NAV1.8 Voltage-Gated Sodium Channel / chemistry
  • NAV1.8 Voltage-Gated Sodium Channel / genetics*
  • NAV1.8 Voltage-Gated Sodium Channel / metabolism
  • Rats
  • Rats, Wistar
  • Sequence Alignment


  • NAV1.8 Voltage-Gated Sodium Channel
  • SCN10A protein, human
  • Scn10a protein, mouse
  • Scn10a protein, rat