Effects of Lactobacillus rhamnosus on allergic march model by suppressing Th2, Th17, and TSLP responses via CD4(+)CD25(+)Foxp3(+) Tregs

Clin Immunol. 2014 Jul;153(1):178-86. doi: 10.1016/j.clim.2014.04.008. Epub 2014 Apr 24.

Abstract

Allergic march (AM) is characterized by the progression of clinical signs of atopic dermatitis (AD) to allergic asthma or rhinitis, but its pathogenesis is not completely understood. We developed mouse model of AM with three 1-week exposures (separated by 2-week interval) to an OVA or saline (control) followed by OVA challenge. The development of AM was confirmed by phenotypes of AD and allergic asthma. Increases in IL-4, IL-17, and thymic stromal lymphopoietin (TSLP) responses were associated with the progression of AM, and these responses were suppressed by treatment with Lcr35. Moreover, Lcr35 treatment led to an increase in the number of CD4(+)CD25(+) Foxp3(+) regulatory T (Treg) cells in the mesenteric lymph nodes (MLNs) of AM mice. In conclusion, the oral application of Lcr35 prevented the development of AM in this model by suppressing Th2, Th17, and TSLP responses via a mechanism that may involve CD4(+)CD25(+)Foxp3(+) Tregs in MLNs.

Keywords: Allergic march; Probiotics; Regulatory T cells; Th17; Thymic stromal lymphopoietin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Asthma / immunology*
  • Asthma / metabolism
  • Asthma / pathology
  • Cytokines / immunology*
  • Cytokines / metabolism
  • Dermatitis, Atopic / immunology*
  • Dermatitis, Atopic / metabolism
  • Disease Models, Animal
  • Female
  • Forkhead Transcription Factors / metabolism
  • Interleukin-17 / metabolism
  • Interleukin-2 Receptor alpha Subunit / metabolism
  • Interleukin-4 / metabolism
  • Lactobacillus rhamnosus / immunology*
  • Lymph Nodes / immunology
  • Lymph Nodes / metabolism
  • Mesentery
  • Mice
  • Neutrophils / immunology
  • Probiotics / administration & dosage
  • Skin / immunology
  • Skin / metabolism
  • T-Lymphocytes, Regulatory / immunology*
  • T-Lymphocytes, Regulatory / metabolism
  • Th17 Cells / immunology*
  • Th17 Cells / metabolism
  • Th2 Cells / immunology*
  • Th2 Cells / metabolism

Substances

  • Cytokines
  • Forkhead Transcription Factors
  • Foxp3 protein, mouse
  • Interleukin-17
  • Interleukin-2 Receptor alpha Subunit
  • Interleukin-4
  • thymic stromal lymphopoietin