Estimation of the toxicity of silver nanoparticles by using planarian flatworms

Altern Lab Anim. 2014 Mar;42(1):51-8. doi: 10.1177/026119291404200108.


The regeneration of planarian flatworms - specifically, changes to the area of the regeneration bud (blastema) after surgical dissection - was proposed for use as a robust tool for estimating the toxicity of silver nanoparticles. The use of Planaria species, due to their unique regenerative capacity, could result in a reduction in the use of more-traditional laboratory animals for toxicity testing. With our novel approach, silver nanoparticles were found to be moderately toxic to the planarian, Girardia tigrina.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Metal Nanoparticles / toxicity*
  • Particle Size
  • Planarians / drug effects*
  • Planarians / physiology
  • Regeneration
  • Silver / toxicity*


  • Silver