Trenbolone acetate metabolites promote ovarian growth and development in adult Japanese medaka (Oryzias latipes)

Gen Comp Endocrinol. 2014 Jun 1;202:1-7. doi: 10.1016/j.ygcen.2014.04.011. Epub 2014 Apr 26.

Abstract

Trenbolone acetate, a synthetic androgen, has been used as a growth promoter in beef cattle in the US since 1987. While several teleost studies have investigated the masculinization effects of the metabolite 17β-trenbolone, few have focused on the reproductive impacts of all three trenbolone acetate (TBA) metabolites including trendione. Adult female medaka (Oryzias latipes) were exposed to TBA metabolites (10, 100, and 1000ng/L) for 14days (n=3). Histological examination revealed that TBA metabolites (1000ng/L) significantly reduced the percentage of primary ovarian follicles and increased the percentage of vitellogenic follicles compared to control fish. 17α-Trenbolone significantly increased whereas trendione reduced whole body levels of estradiol-17β. Testosterone was significantly reduced by trendione treatment and only the highest dose of 17β-trenbolone and lowest dose of trendione altered 11-ketotestosterone. Additionally, TBA metabolites may be further broken down and/or metabolized or converted by the animal influencing both sex steroid levels and ovarian development.

Keywords: Aquatic toxicology; Reproduction; Sex steroids; Trenbolone acetate metabolites.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Biological Assay
  • Biotransformation / drug effects
  • Cattle
  • Estrenes / pharmacology
  • Female
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / metabolism
  • Oncorhynchus mykiss
  • Oryzias* / growth & development
  • Oryzias* / metabolism
  • Ovarian Follicle / drug effects
  • Ovarian Follicle / growth & development
  • Ovarian Follicle / metabolism
  • Ovary / cytology
  • Ovary / drug effects*
  • Ovary / growth & development*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Steroids / metabolism
  • Trenbolone Acetate / metabolism*
  • Trenbolone Acetate / pharmacology*
  • Vitellogenins / genetics
  • Vitellogenins / metabolism

Substances

  • Estrenes
  • RNA, Messenger
  • Steroids
  • Vitellogenins
  • trendione
  • Trenbolone Acetate