Enrichment of cells with TALEN-induced mutations using surrogate reporters

Methods. 2014 Aug 15;69(1):108-17. doi: 10.1016/j.ymeth.2014.04.012. Epub 2014 Apr 26.


Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection.

Keywords: Enrichment; Flow cytometry; Hygromycin B; Magnetic separation; Mutant cells; Transcription activator-like effector nucleases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Separation / methods*
  • Cinnamates
  • Deoxyribonucleases / chemistry
  • Deoxyribonucleases / genetics
  • Flow Cytometry / methods*
  • Genes, Reporter*
  • HEK293 Cells
  • Humans
  • Hygromycin B / analogs & derivatives
  • Magnetics
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Mutation


  • Cinnamates
  • Hygromycin B
  • hygromycin A
  • Deoxyribonucleases