Molecular genetics of pecanex, a maternal-effect neurogenic locus of Drosophila melanogaster that potentially encodes a large transmembrane protein

Dev Biol. 1989 Nov;136(1):1-16. doi: 10.1016/0012-1606(89)90127-9.


In the absence of maternal expression of the pecanex gene, the embryo develops severe hyperneuralization similar to that characteristic of Notch mutant embryos. We have extended a previous molecular analysis of the chromosomal interval that encompasses pecanex by using additional deficiencies to localize the locus on the molecular map. RNA blot analysis shows that the locus encodes a rare 9-kb transcript as well as minor transcripts of 3.7 and 2.3 kb. The temporal expression of these transcripts is appropriate for a neurogenic locus. Phenocopies of the mutant phenotype have been produced following microinjection of antisense RNA corresponding to a portion of the pecanex transcripts. Conceptual translation of a partial coding sequence compiled from cDNA and genomic clones indicates that the pecanex locus potentially encodes a large, membrane-spanning protein.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Central Nervous System / embryology
  • DNA / genetics
  • DNA, Recombinant
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Gene Expression
  • Male
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Mutation
  • Nucleic Acid Hybridization
  • RNA / genetics
  • RNA Probes
  • RNA, Antisense
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic


  • DNA, Recombinant
  • Membrane Proteins
  • RNA Probes
  • RNA, Antisense
  • RNA
  • DNA

Associated data

  • GENBANK/M25662