Treatment of autoimmune MRL/lpr mice with anti-B220 monoclonal antibody reduces the level of anti-DNA antibodies and lymphadenopathies

Immunology. 1989 Oct;68(2):204-8.


The predominant accumulating cells in the lymphoid tissue of MRL/lpr mice have been shown to carry the B220 cell marker. This antigen is expressed on B cells and on T-cell precursors. In order to know the pathogenic involvement of cells carrying this marker, we treated MRL/lpr mice periodically with RA3-6B2, a specific anti-B220 monoclonal antibody, or rat IgG2b as a control. After 12 weeks of treatment, a significant reduction (P less than 0.01) in the size of the lymph nodes and spleen was observed only in the group treated with RA3-6B2 monoclonal antibody. This reduction was mainly due to a decrease in the Thy-1.2+ and B220+ subpopulations. Anti-DNA and anti-Sm antibody titres were also reduced significantly (P less than 0.01) after the therapy. Proliferative response to mitogens (Con A, PHA, LPS) and IL-2 production was not improved after the anti-B220 treatment. These results suggest a pathogenic role of lymphocytes carrying the B220 marker in the autoimmune disease of MRL/lpr mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Antinuclear / analysis*
  • Antibodies, Monoclonal / immunology*
  • Autoimmune Diseases / immunology*
  • DNA / immunology
  • Female
  • Leukocyte Common Antigens
  • Lupus Erythematosus, Systemic / immunology
  • Lymphatic Diseases / immunology*
  • Lymphocyte Activation
  • Membrane Glycoproteins / immunology
  • Mice
  • Mice, Inbred Strains
  • T-Lymphocytes / immunology


  • Antibodies, Antinuclear
  • Antibodies, Monoclonal
  • Membrane Glycoproteins
  • DNA
  • Leukocyte Common Antigens