Real-Time Detection of HIV-2 by Reverse Transcription-Loop-Mediated Isothermal Amplification

J Clin Microbiol. 2014 Jul;52(7):2674-6. doi: 10.1128/JCM.00935-14. Epub 2014 Apr 30.

Abstract

Currently, there are no FDA-approved nucleic acid amplification tests (NAATs) for the detection or confirmation of HIV-2 infection. Here, we describe the development of a real-time assay for the detection of HIV-2 DNA and RNA using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) and the ESEQuant tube scanner, a portable isothermal amplification/detection device.

MeSH terms

  • Equipment and Supplies*
  • HIV Infections / diagnosis*
  • HIV-2 / genetics
  • HIV-2 / isolation & purification*
  • Humans
  • Molecular Diagnostic Techniques / instrumentation
  • Molecular Diagnostic Techniques / methods*
  • Nucleic Acid Amplification Techniques / instrumentation
  • Nucleic Acid Amplification Techniques / methods*
  • Reverse Transcription
  • Virology / instrumentation
  • Virology / methods*