Towards error-free profiling of immune repertoires

Nat Methods. 2014 Jun;11(6):653-5. doi: 10.1038/nmeth.2960. Epub 2014 May 4.


Deep profiling of antibody and T cell-receptor repertoires by means of high-throughput sequencing has become an attractive approach for adaptive immunity studies, but its power is substantially compromised by the accumulation of PCR and sequencing errors. Here we report MIGEC (molecular identifier groups-based error correction), a strategy for high-throughput sequencing data analysis. MIGEC allows for nearly absolute error correction while fully preserving the natural diversity of complex immune repertoires.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Fingerprinting / methods*
  • DNA Fingerprinting / standards
  • High-Throughput Nucleotide Sequencing / standards*
  • Polymerase Chain Reaction / standards
  • Receptors, Antigen, T-Cell / genetics*
  • Research Design*


  • Receptors, Antigen, T-Cell