Monoclonal antibodies directed against basic fibroblast growth factor which inhibit its biological activity in vitro and in vivo

Biochem Biophys Res Commun. 1989 Oct 31;164(2):736-43. doi: 10.1016/0006-291x(89)91521-0.

Abstract

A panel of four murine monoclonal IgG1 antibodies (mAbs) to a recombinant form of basic fibroblast growth factor (bFGF) was produced using somatic cell fusion techniques. Non-linear regression analysis of radioimmunoassay data for each mAb yielded the following dissociation constants (nM) for their interactions with bFGF: DE6 (0.822); AF11 (2.0); FE8 (2.31); and DG2 (20.0). One of the mAbs, DG2, was identified as a bFGF neutralizing antibody on the basis of its ability to inhibit, in vitro, the binding of [125I]-bFGF to high and low affinity bFGF sites on cultured baby hamster kidney cells and bFGF-induced [3H]-thymidine incorporation in cultured 3T3 cells, and in vivo, the angiogenic response to bFGF in a rat kidney capsule model of angiogenesis. The other mAbs displayed varying inhibitory activities in these assays. These mAbs, particularly DG2, may be well suited for a number of applications in bFGF research including immunoassays, immunohistochemical studies, and as functional antagonists of bFGF for examining its role in physiological processes such as reproduction, growth, and development.

MeSH terms

  • Animals
  • Antibodies, Monoclonal* / biosynthesis
  • Antigen-Antibody Complex
  • Binding, Competitive
  • Cell Division / drug effects
  • Cell Line
  • Cells, Cultured
  • DNA Replication / drug effects
  • Female
  • Fibroblast Growth Factors / immunology
  • Fibroblast Growth Factors / metabolism*
  • Fibroblast Growth Factors / pharmacology
  • Kinetics
  • Male
  • Mice
  • Mice, Inbred BALB C / immunology
  • Neovascularization, Pathologic
  • Rats
  • Rats, Inbred Strains
  • Receptors, Cell Surface / metabolism*
  • Receptors, Fibroblast Growth Factor
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / pharmacology
  • Renal Circulation / drug effects

Substances

  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Receptors, Cell Surface
  • Receptors, Fibroblast Growth Factor
  • Recombinant Proteins
  • Fibroblast Growth Factors