Fluorescence properties of selected benzo[c]phenanthridines

J Fluoresc. 2014 Jul;24(4):1177-82. doi: 10.1007/s10895-014-1398-8. Epub 2014 May 6.


The fluorescence properties of selected benzo[c]phenanthridines (BPs) were examined. The effect of structure, pH and solvent on the fluorescence properties has been investigated. It was found out that the presence of charged iminium nitrogen significantly decreased the fluorescence of the compounds. The fluorescence (intensity as well as emission spectra shape) of the investigated compounds was significantly dependent on pH as well as used solvent. The utilization in epigenetic modification mechanisms studies as demethylase probe and as possible pH indicator was suggested.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzophenanthridines / chemical synthesis
  • Benzophenanthridines / chemistry*
  • Flow Cytometry
  • Fluorescence*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Hydrogen-Ion Concentration
  • Molecular Structure
  • Solvents / chemistry
  • Spectrometry, Fluorescence


  • Benzophenanthridines
  • Fluorescent Dyes
  • Solvents