CRISPR transcriptional repression devices and layered circuits in mammalian cells

Nat Methods. 2014 Jul;11(7):723-6. doi: 10.1038/nmeth.2969. Epub 2014 May 5.


A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
  • Gene Expression Regulation
  • Gene Targeting
  • Genetic Engineering / instrumentation*
  • Genetic Engineering / methods
  • HEK293 Cells
  • Humans
  • Promoter Regions, Genetic
  • RNA Polymerase II / genetics
  • RNA Polymerase III / genetics
  • RNA, Small Untranslated
  • Transcription, Genetic / drug effects


  • RNA Polymerase II
  • RNA Polymerase III
  • RNA, Small Untranslated