Investigating spermatogenesis in Drosophila melanogaster

Methods. 2014 Jun 15;68(1):218-27. doi: 10.1016/j.ymeth.2014.04.020. Epub 2014 May 2.


The process of spermatogenesis in Drosophila melanogaster provides a powerful model system to probe a variety of developmental and cell biological questions, such as the characterization of mechanisms that regulate stem cell behavior, cytokinesis, meiosis, and mitochondrial dynamics. Classical genetic approaches, together with binary expression systems, FRT-mediated recombination, and novel imaging systems to capture single cell behavior, are rapidly expanding our knowledge of the molecular mechanisms regulating all aspects of spermatogenesis. This methods chapter provides a detailed description of the system, a review of key questions that have been addressed or remain unanswered thus far, and an introduction to tools and techniques available to probe each stage of spermatogenesis.

Keywords: Cytokinesis; Drosophila; Germ line; Meiosis; Spermatogenesis; Stem cell.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Differentiation / genetics*
  • Cell Movement / genetics
  • Developmental Biology / methods
  • Drosophila
  • Gene Expression Regulation, Developmental
  • Male
  • Spermatogenesis / genetics*
  • Transcription, Genetic*