Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins

Jun Yamamoto; Masaya Denda; Nami Maeda; Miku Kita; Chiaki Komiya; Tomohiro Tanaka; Wataru Nomura; Hirokazu Tamamura; Youichi Sato; Aiko Yamauchi; Akira Shigenaga; Akira Otaka

doi:10.1039/c4ob00622d

Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins

Org Biomol Chem. 2014 Jun 21;12(23):3821-6. doi: 10.1039/c4ob00622d.

Authors

Jun Yamamoto¹, Masaya Denda, Nami Maeda, Miku Kita, Chiaki Komiya, Tomohiro Tanaka, Wataru Nomura, Hirokazu Tamamura, Youichi Sato, Aiko Yamauchi, Akira Shigenaga, Akira Otaka

Affiliation

¹ Institute of Health Biosciences and Graduate School of Pharmaceutical Sciences, The University of Tokushima, Shomachi, Tokushima 770-8505, Japan. shigenaga.akira@tokushima-u.ac.jp aotaka@tokushima-u.ac.jp.

PMID: 24806338
DOI: 10.1039/c4ob00622d

Abstract

A traceable linker that is potentially applicable to identification of a target protein of bioactive compounds was developed. It enabled not only thiol-induced cleavage of the linker for enrichment of the target protein but also selective labelling to pick out the target from contaminated non-target proteins for facile identification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amides / chemistry
Amino Acids / chemistry*
Click Chemistry / methods*
Electrophoresis, Polyacrylamide Gel
Peptides / chemistry
Proteins / chemistry*
Staining and Labeling*
Sulfhydryl Compounds / chemistry*

Substances

Amides
Amino Acids
Peptides
Proteins
Sulfhydryl Compounds