Role of substance P in immediate-type skin reactions induced by staphylococcal enterotoxin B in unsensitized monkeys

J Allergy Clin Immunol. 1989 Dec;84(6 Pt 1):880-5. doi: 10.1016/0091-6749(89)90383-7.

Abstract

The staphylococcal enterotoxin B (SEB)-induced immediate-type skin reaction in unsensitized monkeys was used as a nonimmunologic mast cell stimulation to search for possible involvement of local neural mechanisms. Evidence is presented that substance P (SP) plays a predominant role in mediating intradermal SEB challenge in unsensitized monkeys. With a rabbit SP antiserum directed against the C-terminal region of SP, a concentration-dependent inhibition of SEB-induced skin reactivity could be demonstrated. Furthermore, a rabbit antiserum directed against the mast cell activating N-terminal part of SP was capable of impeding SEB-induced skin reactions totally. By use of SP antagonists, significant reduction of skin reactions evoked by SEB was found. Finally, capsaicin pretreatment of the skin caused a substantial inhibition of SEB-induced skin reactivity. These data suggest that SEB exerts its effect on cutaneous mast cells via stimulation of primary sensory neurons that contain SP. Moreover, a new in vivo model is described for studies of nerve-mast cell interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Capsaicin / pharmacology
  • Enterotoxins / immunology*
  • Hypersensitivity, Immediate / immunology*
  • Immune Sera / immunology
  • Macaca fascicularis
  • Skin / drug effects
  • Skin / immunology*
  • Skin Tests
  • Staphylococcus aureus
  • Substance P / antagonists & inhibitors
  • Substance P / immunology*

Substances

  • Enterotoxins
  • Immune Sera
  • Substance P
  • enterotoxin B, staphylococcal
  • Capsaicin