The EF-hand calcium-binding protein tescalcin is a potential oncotarget in colorectal cancer

Oncotarget. 2014 Apr 30;5(8):2149-60. doi: 10.18632/oncotarget.1851.

Abstract

Tescalcin (TESC) is an EF-hand calcium binding protein that is differentially expressed in several tissues, however it is not reported that the expression and functional roles of TESC in colorectal cancer. Levels of messenger RNA (mRNA) and protein expression of TESC in colorectal cancer tissues were assessed using RT-PCR, real time PCR, immunohistochemistry, and clinicopathologic analyses. Quantitative analysis of TESC levels in serum specimens was performed using sandwich ELISA. Colorectal cancer cells transfected with TESC small interfering RNA and short hairpin RNA were examined in cell proliferation assays, phospho-MAPK array, and mouse xenograft models. Here we demonstrated that TESC is overexpressed in colorectal cancer (CRC), but was not expressed in normal mucosa and premalignant dysplastic lesions. Furthermore, serum TESC levels were elevated in patients with CRC. Knockdown of TESC inhibited the Akt-dependent NF-κB pathway and decreased cell survival in vitro. Depletion of TESC reduced tumor growth in a CRC xenograft model. Thus, TESC is a potential diagnostic marker and oncotarget in colorectal cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / mortality
  • Adenocarcinoma / pathology
  • Adult
  • Aged
  • Animals
  • Biomarkers, Tumor / analysis*
  • Blotting, Western
  • Calcium-Binding Proteins / metabolism*
  • Colorectal Neoplasms / metabolism*
  • Colorectal Neoplasms / mortality
  • Colorectal Neoplasms / pathology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Heterografts
  • Humans
  • Immunohistochemistry
  • Immunoprecipitation
  • Kaplan-Meier Estimate
  • Male
  • Mice
  • Mice, Nude
  • Microscopy, Confocal
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Up-Regulation

Substances

  • Biomarkers, Tumor
  • Calcium-Binding Proteins
  • TESC protein, human